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It is apparent that in both groups of mice, only the female NZB/WF1 mice demonstrated detectable levels of anti-dsDNA autoantibodies by 18 wks of age.
Filipin labelling in Slc9a6−/− mice demonstrated detectable amounts of unesterified cholesterol in cells of the basolateral amygdala in a manner similar to that observed for GM2 ganglioside (Fig. 4).
Similar(58)
However, RPAs from spleens of control mice demonstrated barely detectable levels for caspase 3 mRNA.
RPAs from spleens of control mice demonstrated barely detectable levels for caspase 2, 3 and 8 mRNA, but caspase 1, 6, 7, 11 and 12 mRNA were not detected.
Whole body image analysis of mice at the time of sacrifice demonstrated that 37.5% of vehicle or daidzein-fed mice demonstrated easily detectable lymph node metastases while only 25% of genistein fed mice demonstrated lymph node metastases, although these findings were not statistically significant when compared to the vehicle treated mice (data not shown).
As few exceptions, the non-proliferating hepatocytes in the adult liver and purkinje cells of the cerebellum also demonstrated detectable levels of β-gal staining in the PZU mice.
Western blot analyses (Figure 12) and ELISA performed on sera from the immunized mice demonstrated that all had IgG and IgM reactivity to the capsule and no detectable reactivity to F. tularensis LPS.
All mice demonstrated full visual capability.
Three mice demonstrated subarachnoidal hemorrhages.
Analysis of cholesterol and gangliosides did not demonstrate detectable storage reductions between the allopregnanolone-treated Npc1−/− mice versus their respective vehicle-injected controls.
Nuclear p53 signal was not detectable in cisplatin-treated p53−/− mice, demonstrating specificity of this immunohistochemistry (IHC) assay.
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