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Mice capable of inducible nephron-specific ETA knockout (KO) were achieved by breeding mice containing the Pax8-rtTA and LC-1 transgenes with mice containing loxP-flanked (floxed) exons 6 8 of the EDNRA gene [ 7].
As expected, only mice containing the AD-related mutations in APP and PS1 were positive for Aβ-containing plaques.
In the cell cultures and living mice containing the ribozyme sequence, protein production dropped to nearly undetectable levels.
This study aimed to generate mutant mice containing the Acvrl1 gene flanked with LoxP sequences to allow conditional deletion of Acvrl1 by the LoxP/Cre system.
Such incomplete resolution of this complex locus occurred progressively with age in germ cells of male mice; the conditional Cyp1a1 gene was recovered in offspring from mice containing the targeted Cyp1a1 allele and the Cre recombinase transgene.
Moreover, semen of the NGO-treated mice (containing the damaged spermatozoa) might disturb the hormone secretion and pregnant functionality of female mice (∼44, 35 and 59% reduction in fertility, gestation ability and multi-production capability) and also viability of the next generation (∼15% reduction in postnatal viability of delivered pups).
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He notes that Israelis have resisted "Maus," uncomfortable that "the image of mice contains the stereotype of Jews as pathetic and defenseless creatures".
Genotyping PCR demonstrated that eggs from mutant mice contained the Separase mutation (Figure 1D).
The mice contained the fusion protein of PER2 and luciferase.
Furthermore, 40% of the F1 mice contained the construct, reflecting a very efficient transmission.
These transgenic mice contain the tamoxifen-inducible Cre gene (CreER) under the control of the keratin 14 promoter.
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