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Genotyping PCR demonstrated that eggs from mutant mice contained the Separase mutation (Figure 1D).
The genomic DR4-H2E transgenes used for creation of DR4 mice contained the murine class II promoter and locus control regions (LCR), enabling natural expression of the chimeric proteins in the appropriate cells and at the appropriate stages of development, as expected for a normal H2 class II molecule [12].
The mice contained the fusion protein of PER2 and luciferase.
Furthermore, 40% of the F1 mice contained the construct, reflecting a very efficient transmission.
Whole-genome sequencing and in vitro hemolytic assays indicated that the microbiota of WT and Il22ra1−/− mice contained the same E. faecalis isolate and that this isolate was recovered repeatedly in the feces, livers, and spleens of Il22ra1−/− mice (Table S4B).
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Mice capable of inducible nephron-specific ETA knockout (KO) were achieved by breeding mice containing the Pax8-rtTA and LC-1 transgenes with mice containing loxP-flanked (floxed) exons 6 8 of the EDNRA gene [ 7].
As expected, only mice containing the AD-related mutations in APP and PS1 were positive for Aβ-containing plaques.
He notes that Israelis have resisted "Maus," uncomfortable that "the image of mice contains the stereotype of Jews as pathetic and defenseless creatures".
As described by [6], fictive sighs occur periodically in the in vitro transverse medullary slice preparation from mice containing the pre-BötC within the VRG.
In the new experiments, researchers from VU University in Amsterdam, the Netherlands, used lab mice containing the deactivated fmr1 gene.
This study aimed to generate mutant mice containing the Acvrl1 gene flanked with LoxP sequences to allow conditional deletion of Acvrl1 by the LoxP/Cre system.
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