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Initial characterization of JAM-C SC KO mice confirmed specific ablation of JAM-C in nerves and its lack of expression at junctional sites in SCs.
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The uncontrolled growth of Renca-VC tumors, compared with Renca-LMP2A tumors, in immunized mice confirmed the specific CTL targeting of LMP2A epitopes expressed by the Renca-LMP2A tumors.
Immunoblot confirmed specific and efficient Med23 deletion in the liver of LMKO mice but not in skeletal muscle or adipose tissue.
Besides, in vivo experiments using endothelial cell-specific MeCP2 null or Sirt1 transgenic mice confirmed the involvement of MeCP2/Sirt1 in the regulation of angiogenic functions of endothelial cells.
However, no detectable ß-actin signal was present in motor neuron cell bodies from Actb-MNsKO mice, confirming motor neuron specific ablation of ß-actin (Figure 1F bottom).
To confirm specific loss of motor neurons in DKO mice, we examined the spinal roots of the lumbar spinal cord by electron microscopy.
To confirm specific uptake of 99mTc-rCR2 in the transplanted hearts, mice were euthanized after imaging and organs removed for gamma counting and histology.
At this dilution, no background immunoreactivities were detected in the brain of the non-TG mice, confirming that 1C2 immunoreactivity is specific to the expanded polyQ stretches of mutant proteins (Fig. 4A, right panels) (10).
In addition, nine of the ten metabolism-related genes examined were also downregulated in LV of the cardiomyocyte-specific KO mice, confirming a cell-autonomous function of NELF-B in gene regulation.
The introduction of specific RTT mutations into mice confirms the value of this model system in studying the molecular basis of this condition.
These results confirm the specific lack of autoantibodies directed against the Sm/RNP complex in serum from pristane-treated IFNAR2-/ mice, confirming the data obtained using autoantigen microarrays.
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