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For their experiments, they created lines of mutant mice by inserting bits of foreign DNA.
In recent experiments, we created a conditional Shp2 knockout (Shp2flox) allele in mice by inserting two loxP sites into introns that flank exon 4 of Shp2.
We generated Gsdma KO mice by inserting the LacZ reporter gene at the start codon of Gsdma.
Saliva was collected from mice by inserting a disposable inoculation loop into the animal's mouth, allowing the animal to chew for a few seconds, before rinsing the loop in QIAamp DNA Investigator Kit ATL buffer.
(A ) Gene trapping was used to generate membralin KO mice by inserting a trapping vector that contained a splicing acceptor sequence between exon 1 and 2 to disrupt normal RNA splicing.
In vivo intraperitoneal pH measurement was carried out in parallel in three of the same anesthetized T47D tumor-bearing mice by inserting the micro pH electrode into the peritoneal cavity through a skin incision.
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We generated a new VAChT targeted mouse line by inserting a lox-P flanked TK-Neo cassette in the 3' region of the VAChT gene, in the intron between exons N and M of the ChAT gene, and a third lox-P sequence 260 bp upstream from the VAChT translational initiation codon (Figure 1).
Anesthetized mice were intubated by inserting a sterile, 20-gauge intravenous cannula through the vocal cords into the trachea.
In today's issue of Science, researchers show that the same protein that causes cows to keel over can, with a small mutation, kill mice in weeks by inserting itself into the membranes of their brain cells.
RAG−/− mice were produced by inserting a targeted mutation on chromosome 2, resulting in a "non-leaky" severe combined immune deficiency[12].
Ghr/bp gene-disrupted (GHR-KO) mice were generated by inserting a neomycin cassette replacing the 3′-end of the fourth exon and the 5′-end of intron 4/5 of the genomic sequence [ Zhou et al., 1997].
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