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We excluded the possibility of malabsorption in Rcan2−/− mice by calculating apparent absorption efficiency.

We next examined the proportion of striatal MSNs which expressed EGFP in the striatum of drd1a-EGFP/drd2-EGFP double transgenic mice, by calculating the percentage of DARPP-32-positive cells that showed EGFP fluorescence.

The Affymetrix "present" tagged probe sets were then ranked as "upregulated" and "downregulated" between wild type and Cln1/5 knock out mice by calculating median fold changes.

We further analysed onset latencies in control mice by calculating signal changes greater than two SDs of baseline fluctuations (Fig. 13), as was done in Experiment 1. Negative responses were not consistently observed in layer I and there were no differences in onset between the other layers (F3,12 = 1.7; P = 0.2).

We verified the dynamics of fuel selection in AL and CR mice by calculating the respiratory exchange ratio (RER) between the amount of CO2 exhaled and O2 inhaled from mice kept individually in metabolic chambers (RER = VCO2/VO2 = 1.0 for pure carbohydrate usage; RER = 0.7 for pure fat usage) (see Fig EV2A and legend for further explanation).

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The sizes (e.g. cephalad-caudad length) were calculated from mouse segmentations by calculating the number of transaxial slices containing the mouse and multiplying this number by the slice thickness.

The homozygosity of Tg mice was determined by calculating the ΔCt using real-time PCR with primers for EGFP (test gene) and ApoB (reference gene).

We recorded spectral irradiance profiles in horizontal view over a dusk transition in an urban setting (solar angles from −9° to 30° under clear skies but outside of direct sunlight; Figures 1A and 1B) and modeled the mouse's experience of these conditions by calculating the effective photon flux for each of the mouse photopigments.

We evaluated the sequence conservation of the miRNAs based on the whole genome alignments of human, rhesus, mouse and 14 other vertebrate species by calculating the per-site conservation probability of the human pre-miRNAs.

Virulence was assessed in previous studies by calculating the lethal dose that killed 50% of mice (LD50).

In vivo cytotoxicity was assessed by calculating the ratio of unpulsed/pulsed targets in immunized versus naive mice.

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