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A sensitive and selective method was elaborated for the determination of the anticonvulsant compounds levels in mice brain tissue, based on HPLC with diode array detector (DAD).
Next, we evaluated XIAP expression in nude mice brain tissue lysates by immunoblotting.
In hUCBSC-treated mice brain tissue, the presence of hUCBSC as confirmed by mesenchymal stem cells markers CD29 and CD81, clearly establishes the fact that hUCBSC are responsible for tumor size reduction observed in the hUCBSC-treated brains (Figs. 4B and 4C).
Paraffin-embedded sections (6 µm) of mice brain tissue were deparaffinized and hydrated followed by antigen retrieval with boiled 0.01 M citrate buffer, pH 6.0, for 10 min. Brain sections were immunostained with anti-GFAP, anti-Iba-1 or anti-LC3 antibodies.
Biological sample used here was total protein prepared either from the IPTG-induced BL21 or from fresh mice brain tissue.
Immunohistochemical analysis of PS2APP transgenic mice brain tissue 3 days after intravenous injection of gantenerumab showed dose-dependent binding to amyloid plaques.
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Normal and tumor mice brain tissues were collected after treatment with saline, vardenafil, Herceptin, and Herceptin plus vardenafil.
Nine-month-old male P301S mice brain tissues were homogenized in TBS supplemented with protease and phosphatase inhibitors (Roche, Indianapolis, IN) as described previously.
Changes in affinity might be caused by differences in the secondary structures of Aβ peptides deposited in human and mice brain tissues.
VEGFR2 protein levels are significantly decreased in transgenic mouse brain tissue.
However, immunohistochemical staining of 12C-irradiated mouse brain tissue showed 2 months later a marked inflammatory reaction in the hippocampus and a diffuse response in parenchyma.
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