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For mice brain sections (20 µm), brains were frozen and cut with CryoStar NX70 cryostat (ThermoFisher Scientific).
[18F]9a clearly labeled Aβ plaques in in vitro autoradiography of postmortem AD patients and Tg mice brain sections.
For mice brain sections, cornu ammonis (CA) 1 3 areas were traced at low magnification since the icv albumin is infused into these areas of hippocampus as previously shown6.
After deparaffinization and hydration, mice brain sections were stained with 0.1% cresyl violet solution.
Further, we evaluated PTEN expression in nude mice brain sections by colocalization studies using PTEN and CD81 antibodies.
For TUNEL assay of mice brain sections, a similar procedure was followed after deparaffinization of the sections.
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De Waele, J. et al. 3D culture of murine neural stem cells on decellularized mouse brain sections.
Furthermore, in vitro autoradiography studies of [18F]-9 with AD brain tissue sections and ex vivo autoradiography studies in transgenic mouse brain sections show cortical Aβ binding, and a fair correlation with Aβ immunostaining.
Fig. 5 a Representative coronal S1R-stained WT and S1R-KO mouse brain sections.
S1R-KO mouse brain sections were found to be devoid of S1R staining.
Comparative studies of antiquitin distribution were performed in developing mouse brain sections.Histologic analysis of PDE cortex revealed areas of abnormal radial neuronal organization consistent with type Ia focal cortical dysplasia.
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