We analyzed exon array data for a panel of mouse tissues, (brain, embryo, heart, kidney, liver, lung, muscle, ovary, spleen, testes and thymus), each with three replicates http://www.affymetrix.com/support/technical/sample_data/exon_array_data.affx.
Sagittal sections of adult BALB/c mouse brain or mouse embryos at different stages of gestation (E9 E16 and P0) were hybridized to DIG-labelled 325 bp sense and antisense RNA probes corresponding to the transcript region encoding the TG6 β-barrels.
With a set of seminal publications, five groups almost in parallel demonstrated the applicability of ChR in hippocampal neurons, PC12 cells, the spine of living chicken embryos, mouse brain slices, the retina of blind mice and transgenic C. elegans.
The four preparations used in the primate studies were all derived from P9605, which had been adapted to MKTC following one infant mouse brain and two chick embryo tissue culture passages.
For specificity, milk antibody was pre-adsorbed to an acetone extract of E16 mouse embryos and adult mouse brain, liver and lung.
Total RNA was extracted from the P0 mouse brain and liver, from whole mouse embryos (E10.5) or from cultured cells by TRI-reagent (Molecular Research Center, Cincinnati, OH, USA) according to the manufacturer's instructions.
»…demonstrated the functionality of ChR2 in the retina of blind mice, hippocampal neurons, spine of living chicken embryos, PC12 cells, mouse brain slices and transgenic worms…« ChRs are composed of seven trans-membrane helices that form the ion channel and a long C-terminal extension of unknown function, which is routinely omitted for optogenetic purposes.
In 1967, formalin-inactivated CHIKV vaccines were prepared in chick embryo and suckling mouse brain in 1967 and 1972, and in green monkey kidney cell tissue culture [ 76, 77].
In addition, three ESTs (AU079352, AU080182 and BY729407) from mouse adult brain and embryo spinal cord map in this intronic region (fig. 1A), suggesting that it contains an alternative promoter with a preference for neural tissue.
Otp−/− mice embryo brains of various stages were embedded in 4 % agarose, cut 100 μm-thick with a vibratome in horizontal plane, and processed as floating sections for in situ hybridization of Ghrh, according to the protocol of Shimamura et al. (1995).
