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They have done this with mice before birth and with mice at four weeks of age.
Recently, layered double hydroxide of magnesium-aluminium was administered to male Balb/c mice at four different doses [32].
Pumps dispensing 150 ng/h leptin were implanted in male ob/ob mice at four weeks of age (hereafter referred to as "ob-norm"), and leptin treatment normalized the body weight of these animals within three weeks (Figure 1b).
Intradermal injection of LL-37 into Balb/c mice at four 12-h intervals for 48 h produced cutaneous erythema with central necrosis (Figure 5A), prominent intradermal PMN infiltration (Figure 5C and Figure 6B), marked edema of the dermis (Figure 5C) and increased tissue myeloperoxidase (MPO) activity (Figure 5E).
To confirm the expression of our clones in the visual cortex, we prepared CAGE libraries [10] [11] from the visual cortex of mice at four developmental stages (P21, P26, P54 and P71) and compared the overlap of the cDNA and CAGE libraries for gene detection.
Overall, the performance of Hdac7+/− mice did not differ from WT [F 1,45) = 0.047, P = 0.83] but did change with age [F 2,900) = 4.427, P = 0.009] however, this is most likely the result of the exceptionally good, but atypical, performance of the WT mice at four weeks.
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The IPGTT was carried out in recipient mice at five days after transplantation.
The formulation was orally administered to mice at two dose levels (D1 and D2).
Conversely, transplanted cKit+ donor HSPCs transduced with an shRNA vector against Nap1l3 resulted in a significant reduction (2 18% of engraftment) of engrafted cells compared to the control mice at two, five, eight and 16 weeks post transplantation (Fig. 2b).
For ex vivo xenograft assay, cells were injected subcutaneously into the left flanks of female NOD/SCID mice at three cell densities (5 × 104, 1 × 104, and 1 × 104) in serum-free DMEM with Matrigel at 1 1 ratio.
Histopathological observations of lungs from all mice at two weeks post challenge revealed healthy lung parenchyma.
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