Sentence examples for mice as template from inspiring English sources

Cre and YFP mRNA specific RNA probes were generated by PCR using cDNA from cartilage of Col10CreYFP + mice as template.

Cloning of the murine GPR17 was achieved by nested PCR using genomic DNA from mice as template and verified by sequencing.

The levels of gene expression were analyzed by semi-quantitative RT-PCR using mRNAs from proximal jejunums of fed and fasted wild type and W/W V mice as templates.

Here we have used AFM to image DNA generated by PCR using genomic DNA isolated from tail snips taken from R6/2 mice as a template.

Furthermore, the RT reaction using host RNA isolated from total blood cells of rabbit and mice as the template also did not produce amplification (lane 1 and 2 in Figure S5B), further confirming that the three novel miRNAs are specific for S. japonicum.

Together, the two Ildr1 primers yield a PCR 464 bp product using genomic DNA from both wild-type and heterozygote mice as a template.

A weak central band was also identified; this was confirmed to be an artifact by repeating the RT-PCR for AR exon 2 using gel-purified combined AR exon 2 PCR products from ARKO and WT mice as a template (i.e., no genomic DNA was included in the reaction, yet the central band was present).

We confirmed the human specificity of our primers by lack of an amplicon using cDNA from healthy non-manipulated mouse liver as template (data not shown).

The pcDNA3-Id3 construct was obtained by cloning the 3' UTR Id3 cDNA region - amplified using genomic mouse DNA as template - into the EcoRI5'-HindIII3' sites of the pcDNA3 vector, and was checked by sequencing.

The pGL3-Id3-prom/-1592 construct was obtained by cloning in the 5'SacI-3'BglI site of pGL3-basic the PCR-amplified region of the Id3 promoter (1592 nt before transcrition start), using genomic mouse DNA as template.

In vitro testing for analysis of the cytotoxicity of the formulations was performed using J-774 mouse macrophages as template.