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These differential changes reflected the differential stress susceptibility of the mice, as evaluated by changes in body weight.
Compared to N AL mice, EOD feeding increased overall lifespan in normal mice as evaluated by log-rank analysis (P<0.008); however, there were no significant diet effects in GHRKO mice (P = 0.6727).
A significant enhancement, by at least a factor of two, of implant size was also found upon implantation of embryonic pig liver in Factor VIII KO SCID mice, as evaluated by ELISA for pig blood albumin levels (Fig. 3D).
Although all animals in both control and experimental groups got infected, the transmission efficiency of Borrelia to mice that received tick-immune sera was decreased when compared to control mice, as evaluated by decreased spirochete burden in the skin (P = 0.04) and bladder (P = 0.05), 21 days after the ticks had detached (Fig 7B).
Changes in joint pathology of ank/ank mice, as evaluated by histologic and radiographic means, are qualitative, but only semiquantitative.
And no significant abnormalities were found in these mice as evaluated pathologic examinations of heart, liver, and kidney tissues.
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The health of the mice was not affected as evaluated by the lack of body weight loss.
In addition to the behavior analyses, αS pathology was remarkably ameliorated in the mice treated with gAPN compared to the mice that received vehicle injection as evaluated by immunohistochemistry; accumulation of pαS was reduced in both cortex and olfactory bulb, while αS-positive globule formation in thalamus was remarkably suppressed (Fig. 3D).
An intraperitoneal glucose tolerance test (IpGTT) performed in adult male animals showed that in ctsk−/− mice, postloading plasma glucose levels, as evaluated by the incremental area over basal values during 120 min (ΔG), were significantly (t = 4.59, df = 14, P<0.01) lower than in WT controls (ΔG = 553.74±73.54 and 1145.78±111.82 mg/l/min in ctsk−/− and WT mice, respectively).
Control mice were bled at diestrus, as evaluated by vaginal smears.
Mechanical allodynia in mice was evaluated as described elsewhere (Labuz et al, 2009) using calibrated von Frey filaments (Stoelting, Wood Dale, IL).
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