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However, to clarify the precise roles of IVA-PLA2 in the liver and adipose tissues, further studies with liver- or adipose-specific IVA-PLA2-knockout mice are needed.
Further analysis of expression of these and other genes now identified as being changed in Neurod1 CKO mice are needed for Neurog1 null mice to fully understand the complexity of interaction of the genes in this developing system.
While the assays of cell invasion reported here are widely used for demonstrating metastatic potential [59], [60], and the results appear convincing, further in situ studies with immunodeficient mice are needed to confirm the role of KCNMA1 in melanoma invasiveness in vivo.
"At a minimum, xenograft studies in mice are needed".
New animal models of chronic pulmonary hypertension in mice are needed.
Further studies involving a larger number of mice are needed to confirm the above conclusion.
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Animal experiment was carried out based on the orthogonal design L8 (27) setting seven factors with two different levels of each, and eight groups of mice were needed.
Two independent transgenic mouse strains, eEF-2K transgenic mice and Cre transgenic mice, were needed (Figure S1).
However, further work using knockout mice is needed to fully elucidate the mechanism of Nek2 downstream signaling in this process.
Only a single generation of mice was needed to make the CMMB, large numbers of G1 mice were generated and euthanized in a short time, and the cost of making the bank was a one-time investment.
To ensure a statistical power of at least 0.8 and an alpha of 0.05, a total of eight mice were needed in each group for the comparison of the two groups.
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