For plasma cell isolation ex vivo from bone marrow, mice were immunised with ovalbumin, followed by a second immunisation after 3 weeks.
DBA/1 mice were immunised with CII in CFA; 13 days after immunisation, the mice were treated every second day with 0.25 mg of anti-CD25 (PC61) or control IgG antibodies, for 4 weeks (injected intraperitoneally).
HHD mice were immunised with individual peptides, splenocytes were re-stimulated in vitro with the immunising peptide, and cytotoxicity against cells loaded with the immunising peptide was measured.
BALB/c mice were immunised with GnRH lipopeptide conjugates, co-administered with or without Complete Freund's Adjuvant, followed by two additional immunisations.
Female C57BL/6 mice were immunised using the rVV-Ag85 constructs, and interleukin-2 and gamma-interferon were assayed using a co-culture of immune splenocytes and recall antigen.
In contrast, when HLA A2/Kb transgenic mice were immunised with these peptide epitopes, CTL responses were observed to all epitopes with a maximal response to the epitopes within the structural proteins and low to moderate responses to the latent epitopes.
Donor B6.SJL mice were immunised with BCG and boosted 2 mo later with BCG or Ag85B-TB10.4.
IFNAR −/− mice were immunised by two intraperitoneal injections of MVA-VP2 administered on days 0 and 28 using 107 pfu/mouse.
HHD-2 mice were immunised with the Ad-gBCMVpoly vaccine (7.5×108 pfu/mouse) and both humoral and cellular immune responses were evaluated at the indicated time points.
OT-II mice were immunised for the indicated periods of time and fed with 5-Bromo-2'-deoxyuridine (BrdU; 800 µg/ml) in drinking water for three days.
