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In the tissues samples stained with haematoxylin and eosin, the brain, heart, and kidney from 8to3737 nm GNP-treated mice appeared indistinguishable from tissues in control mice (data not shown).
The deletion was confirmed by Southern blot, PCR and northern blot analysis (Fig. 1C to E). PiT1Δ5/+ mice appeared indistinguishable from PiT1+/+ littermates, were viable and fertile producing offspring in normal Mendelian ratios when crossed with wild-type animals (Table 1).
In other respects, the rescued mice appeared indistinguishable from their wild-type littermates.
At birth, heterozygous (Atp6v0a4+/−, Het) and homozygous (Atp6v0a4−/−, KO) knockout mice appeared indistinguishable from their wildtype (Atp6v0a4+/+, WT) littermates and were born from heterozygous matings at Mendelian ratio.
Indeed, the SVZ regions of IDS-ko and wt mice appeared indistinguishable (Supplementary Figure 2), supporting the hypothesis that GAG metabolism has no significant influence on NSC self-renewal or early differentiation neither in vitro nor in vivo.
While heterozygous Pitx3WT/GFP mice appeared indistinguishable from wild-type animals at the behavioural and histological level (not shown), the Pitx3GFP/GFP knockouts displayed a phenotype which is very similar, if not identical, to the previously described aphakia mice (Nunes et al., 2003; Smidt et al., 2004).
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Heterozygous Ate1+/− mice appear indistinguishable from their wild-type counterparts, whereas Ate1−/− mice die around embryonic day 15 (E15) with abnormalities that include cardiovascular defects [10].
At E18.5, pancreata of Hhex loxP/loxP Pdx1-Cre EarloxP/loxP Pdx1-Creistologically indistinguishablEarlymiceose of littermappeared loxP/loxP controls (n ≥ 3 animals for eachistologically
Mice homozygous for complete deletion (Tln2cd/cd) appeared indistinguishable from their heterozygous and wild-type littermates.
Whereas the treatment did not improve the glycocalyx exclusion of circulation plasma, it robustly alleviated the compromised glycocalyx barrier for Dex70, as evidenced by the increased Dex70 excluding glycocalyx volume which appeared indistinguishable from that measured in the C57Bl/6 mice.
The same study also reported that mice lacking both AgRP and the potent orexigen neuropeptide Y (NPY) appeared indistinguishable from wild-type littermates, leading these authors to conclude that neither AgRP nor NPY had an essential role in feeding behavior.
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