Sentence examples for mice and single from inspiring English sources
Spleens were isolated from uninfected mice, PbA-infected mice and PbA-infected, AC-treated mice and single cell suspensions generated by passing tissue through a 100 µm cell strainer.
Pancreatic cultures were prepared from P1 NPY-GFP mice and single GFP positive cells were aspirated into 5 10 µm diameter patch pipettes containing 1 µl DEPC-treated water.
Spleen and lymph nodes (inguinal and lumbar) were harvested from 6 8 week old Agneg mice and Agpos mice and single cell suspensions obtained by macerating tissues through a 40 µm nylon cell strainer.
We observed that the ghrelin response system of AGRP/NPY neurons was functionally defective in AGRPPdk1−/− mice and single neurons isolated from AGRPPdk1−/− mice exhibited significantly attenuated changes in [Ca2+]I in response to ghrelin.
The spleens were aseptically removed from the immunized and infected BALB/c mice and single cell suspensions were prepared in RPMI 1640 supplemented with 10% FBS, l00 U/ml penicillin G sodium, 100 µg/ml streptomycin sulfate and 50 µM β-mercaptoethanol (Sigma-Aldrich) (complete medium).
Thymus was dissected from mice and single cell suspension was prepared under aseptic condition.
The Thy/Liv implants were collected from euthanized mice, and single-cell suspensions were prepared by dispersing the implant through nylon mesh and processed for p24 ELISA, bDNA assay, and FACS analysis as described [8], [20].
To determine whether transgenic mice overexpressing Id1 were able to produce milk and feed pups, female bi-transgenic mice and single-transgenic controls were given doxycycline chow at the time of mating and pups observed.
Tyrp1 b Gpnmb R150X mice and single-congenic B6.
Popliteal and inguinal lymph nodes were isolated from mice and single-cell suspensions were incubated with anti-CD4 microbeads (Miltenyi Biotec) and subsequently MACS-purified.
Importantly, the 4xTg mice exhibit accelerated cognitive decline compared with 3xTg-AD mice and single-transgenic SNCA mice, suggesting that the exacerbated pathology is clinically relevant.
