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Therefore, cells were freshly isolated from tg hGFAP/EGFP) mice and selected according to their characteristic morphology and specific immunolabeling (GFP positive, GFAP negative) [18].

P. berghei parasites were transfected with linearized plasmids, using the Nucleofector® device (Amaxa GmbH) as described [44], injected intravenously into naïve NMRI mice, and selected by pyrimethamine treatment in the drinking water.

The linearized targeting vector was electroporated into J1 embryonic stem cells derived from 129/terSv mice, and selected embryonic stem cell clones harbouring homologous recombination (determined by PCR and Southern blotting (not shown) were injected into C57BL/6J blastocysts to produce chimeric mice.

These antibodies were raised against recombinant mouse rmPrP23-231 in PrP-deficient (Prnpo/o) mice and selected for binding to both denatured PrP in Western Blot (Supplementary Figure S1) and native, cell-bound PrPC by flow cytometry and ELISA (Supplementary Figure S2).

We established transgenic mouse lines by backcrossing the founders to B6C3F1 hybrid mice, and selected the mouse line with highest expression levels of WT (tgWT) or N88S/S90L mutant (tgMT) Seipin in the central nervous system (CNS).

We investigated the effects of an array of adjuvants on the immune response in mice, and selected the best three adjuvants for a similar investigation in cattle using recombinant congopain (C2) [ 16] as a model antigen.

Similar(54)

To date, some antibody engineering technologies have been developed to achieve this goal: fully humanized antibodies are derived by immunizing transgenic mice, and selecting the recombinant human antibody native or immunized phage display libraries.

C2C12 myoblasts were originally isolated from wild-type mice [39] and selected for its ability to differentiate to myotubes expressing characteristic muscle proteins [40].

Because gene expression profiles of LSC may differ depending on the stage of the disease, we investigated the LSC population of each leukemic mouse separately and selected for analysis only those mice exhibiting advanced MPD but no blast crisis progression.

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We have also included data from mouse studies and selected studies with various types of perturbations in order to explore the generality of observed correlation patterns.

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