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S. pneumoniae (3 × 108CFU) was intranasally administered to WT mice and maintained for 7 days.
Nscl-2 knockout mice were backcrossed for >10 generation to C57BL/6J mice and maintained on the same background.
Briefly, transverse cerebellar slices (350 µm) were made at 4°C from brains of 30-day-old mice and maintained in artificial CSF 23°C.
The A375SM human melanoma cell line [67] was previously established from pooled lung metastases produced by A375-P cells injected i.v. into nude mice and maintained in Eagle's MEM supplemented with 10% fetal bovine serum (FBS), as previously described [20].
These cells were obtained from skeletal muscles of newborn mice and maintained in culture in the presence of high serum concentration and basic fibroblast growth factor (bFGF) to promote proliferation and prevent differentiation.
Polyclonal neural stem cell lines were established from the E12.5 cortex of Dicer-null and wild-type littermate mice and maintained according to published methods with minor variations [23].
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A source at Yale adds that technicians are being allowed to enter for 2 hours at a time to care for research mice and maintain cell strains.
Also, chronic treatment with rapamycin enhances learning and memory in young mice and maintains memory in old mice, and exerts anxiolytic and antidepressant-like effects [ 25].
Vaccination through the intranasal route in mice induced and maintained long-lived protective immunity against the challenge of Y. pestis (Ulery et al., 2011).
Animals were housed in standard mouse cages and maintained in a 16 L:8D photoperiod at 20 ± 2°C.
After 2min, the microcapillary was retracted, and the mouse sutured and maintained in a thermal blanket until recovered from anesthesia.
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