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As done in other strains OVA sensitization was performed in 8 week-old mice and given the kinetics of the experiment (described above) the challenge was performed one week later (day 7 to 9).
They were then mated with male mice and given BPA-containing drinking water for another week.
Surviving nymphs were infested on 8 naïve mice and given the opportunity to blood feed till drop off.
Furthermore, to confirm whether GHSR is involved in the function of macrophage, peritoneal macrophages were collected from C57/BL6 mice, and given DLS (Bachem, UK) at 20 or 100 μM after LPS 24 hour stimulation.
Functional studies have shown that Xist is clearly the essential trigger in mice and given that it is conserved in eutherians, it is fair to imagine that is would play a similar role, although functional proof of its role in XCI in other mammals has not so far been demonstrated.
It is possible however that there is a role also for the reduced α-granule secretion in these mice, and given that many vasoactive cargoes are released from α-granules, this could also play a role in protection from metastasis in these animals (Harper et al, 2013a).
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TUBO cells grow progressively in normal BALB/c mice and give rise to tumors which are histologically similar to those in BALB NeuT mice.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com