They fed NB-DNJ to six inbred male mice for 5 weeks and caged each with four different female mice each week.
Earn enough money to buy the Mouse and cage.
For each preference test, the mice were caged individually and provided chow ad libitum and two bottles.
Each cage housed three mice, and all cages were located in an animal room with temperature ranging from 68°F to 72°F and 25 75% relative humidity with 16/8-hr light/dark cycle.
To obtain embryos at defined embryonic stages, timed matings were performed in which male and female mice were caged together overnight then separated immediately after the mating plug appeared.
Male mice were caged individually, and daily food consumption was measured for 7 days.
Figure 3b shows the frequency of litters containing dead pups (the exact numbers were normally not possible to count) in breeding cages containing Cia40/Pregq2 congenic female mice and breeding cages containing only wildtype littermate females.
The particular context (stressor applied during preceding dark phase) of the 2 hrs liquid consumption measurement intervals involved overnight illumination (Sunday Monday), wet cage and cage tilt (Tuesday Wednesday and Thursday–Friday, long-term CMS in BL6/J and D2Ola mice) and changed cages (Wednesday Thursday, shorter CMS in BL6/J, BL6/N and the other strains), respectively.
Eight-week-old male mice were individually caged and fed ad libitum.
For the elevated plus-maze (EPM) and open-field tests, male C2CR and control mice were individually caged 48 h prior to the tests, then moved from the holding room to the behaviour room 2 h prior to the tests for acclimatisation.
