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Teflon plates coated with the tested films were inserted subcutaneous in mice and analysis of the population of cells on the surfaces was performed.
In vivo studies were fulfilled by subcutaneous implantation of Teflon plates coated with the tested films in mice and analysis of the population of cells on the surfaces.
Current methods for quantifying beta-cell regeneration require the sacrifice of mice and analysis of pancreatic sections, thus precluding the real-time measurement of beta-cell regeneration.
We were unable to obtain newborn SNAP-23-deficient mice, and analysis of pre-implantation embryos from Snap23Δ/wt matings revealed that Snap23-null blastocysts were dying prior to implantation at embryonic day E3.5.
The guidance on more generic procedures, such as intravenous administration of viruses in mice and analysis of pharmacokinetics are less helpful and could reasonably have been omitted.
Transgenic overexpression of hCAP18 in a low malignant breast cancer cell line promoted the development of metastases in SCID mice, and analysis of hCAP18 transgenic tumours showed enhanced activation of MAPK signalling.
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For example, genetic approaches in the mouse and analysis of human polyposis syndromes and colon cancer suggest that loss of TGFβ/ BMP pathway activation augments the Wnt signalling pathway, which disrupts tissue renewal and drives intestinal polyp/tumour formation.
SB, ML, QL, and VV did western blot analysis of mouse brains and contributed to monitoring of mice, collection and analysis of data.
AE-M carried out phenotyping of the mice, construction and analysis of SAGE libraries, confirmation of SAGE tags, IPA analysis, TGF-β assays, and manuscript preparation.
Long-bone-derived PαS cells and mSSCs are most prevalent in newborn mice, and molecular analysis shows that they constitute distinct progenitor populations from the earliest stages onward.
Necropsy of the mice and histological analysis of the limb musculature did not reveal any lesions or apparent abnormalities.
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