Primary mouse stellate cells freshly isolated from C/EBPβ+/+ mice and activated by collagen type 1 matrix [6], were transfected with vectors expressing RSK wild type, a catalytically inactive, dominant negative RSK mutant, or C/EBPβ-Ala217 together with GFP [10].
Photocaged GFP-loaded UCNPs under the skin of mice and activated using the NIR laser have also been demonstrated.
For the set of 12 differentially expressed genes, GRAIL identified common keywords "induced, complement, phosphatase, induction, activation, response, neurons, expression, dopaminergic, mapk, transcription, kinase, cycle, mitogen, after, midbrain, rats, dopamine, mice, and activated".
After co-culturing for several hours, we found that the CD11ahiFcγRIIIhi B cells, which were isolated from LM-infected mice and activated by CD40 ligation, significantly inhibited intracellular bacterial growth/survival in macrophages in comparison to CD40-activated conventional B cells.
Tconv were isolated from WT or Bim−/− Foxp3GFP reporter mice and activated with either plate-bound anti-CD3 (clone 2C11) and soluble anti-CD28 (clone 37.51) or pre-coated CD3/CD28 beads in the presence of 5 ng/ml TGF- β and 100 U/ml IL-2 for 3 days.
To examine direct effects of inflammatory stimulation on STAT5 expression, LSECs were isolated from wild-type mice and activated ex vivo with TNF-α and IL-1β.
Serine enzyme induced visceral pain sensitivity and intestinal permeability in mouse, and activated the intestinal PAR-2 receptor [ 11].
The pituitary hormone FSH is released in a cyclic manner (monthly in humans and every 4 days in the mouse) and activates a new set of growing ovarian follicles.
Both astrocyte and microglia remained inactivated on day10 in Olig1−/− EAE mice, and when activated, their activation were milder compared with WT EAE mice on day 20 (middle and lower panels in Figure 2A, 2B and 3B, 3C).
Whole deactivated Saccharomyces cerevisiae cells expressing a recombinant fusion of HBV X, S and Core antigens elicit T cell responses in mice and activate human T cells linked with viral clearance.
EGCG stimulates expression of many Nrf2-dependent genes in liver and intestine in mice and activates HO-1 expression in B lymphoblasts, epithelial and endothelial cells [13,14,17,18].
