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They are healthier and less obese than mice allowed to eat all day and night long.
Female mice allowed to choose a friend as a nesting partner will bear more pups than females forced to share straw space with a mouse they dislike.
Importantly, mice allowed to explore their cages had higher numbers of cFos-expressing granule cells than those left in their home cages for the duration of the experiment, and those that received optogenetic stimulation during the exploration had significantly higher numbers of cFos positive neurons than those that did not.
In a separate experiment, the team tested muscle from mice put on a restricted diet at the age of 2 months, amounting to 76% of the calories consumed by mice allowed to eat freely.
Compared with mice allowed to eat high-fat, high-sugar chow whenever they wanted, those given access to the same diet, but only for nine hours a day, gained only half as much weight, even though both groups consumed about the same number of calories.
It was demonstrated that CR started at mid-life in adult mice allowed to preserve strength, coordination and spontaneous alternation behaviour when the mice began to age [12].
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We here show that the expression of GFP in cx3cr1 GFP/+ mice allows to discriminate between microglia and macrophages at early time points after acute insult (24 h).
Polymorphisms between molossinus and domesticus mice allow to distinguish the maternal from the paternal allele.
Once the mouse tail tip was fully within one of the goal arms, the goal arm door was closed and the mouse allowed to explore for 30 s.
During the testing period, mice were allowed to explore both of the objects for 5 minutes.
LDTghM3→VTA mice were allowed to recover for a minimum of 1 week.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com