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The dyad was considered stable when one of the two mice achieved the dominant social rank (i.e., for 3 consecutive days the dominant and subordinate roles did not change).
The five mice achieved the criterion by 21 days for the unilateral stimulation task (broken line in Fig. 1C) and by 46 days for the bilateral stimulation task (solid line in Fig. 1C).
In seven cases, mice achieved the criterion at the end of the third session (taking ∼40 min), whereas in the remaining two experiments, the mice achieved the criterion within a single session.
All mice achieved the stable baseline (Stage 6 of training) performance criteria for testing in the 5-CSRTT.
In contrast, nine BL-10 mice achieved the 180 s maximum, five from the IL-6r mAb group and 4 in the Kh-5 group.
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Although ATRX loss in the cartilage of mice was confirmed by immunohistochemistry, immunofluorescence and western blot analyses, we show that long bones from AtrxCol2Cre mice do not differ from controls in length or growth-plate morphology, and that these mice achieve the same adult length and weight as control littermates.
For the post-training performance on the rotarod, the two strains of mice achieved equal performance – C57BL/6 mice fell off at 25.2 ± 3.8 rpm and 129X1 mice at 24.4 ± 3.1 rpm (P > 0.1).
Two of the four UNO mice achieved thresholds at least half a log unit lower than the best performing control mouse and the other two control mice had the same threshold as the best performing control mouse.
The antitumor treatment occurred with an acceptable toxicity level, and the treated mice achieved long-term survival.
During training, both +/+ and D/+ mice exhibited motor learning, but the mutant mice never achieved the wild-type performance level (Fig. 5A), indicating an intrinsic impairment of motor function.
Thus, beginning the study with 3 month old mice achieved relative stability of lean tissue mass in the control group and allowed diet interventions of sufficient duration while avoiding confounding factors of advanced age.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com