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Following irradiation of one limb in mice, a single dose led to improved exercise capacity for at least 7 months following drug treatment.
In both Nrf2 WT and KO mice, a single dose of UVB (300 mJ/cm) significantly increased p53 protein expression compared to untreated skin.
After the initial tumor was palpated in a second cohort (n=2) of Col1a1 FRT-STOP-FRT-Cre-ER-T2; Kras FRT-STOP-FRT-G12D/+; p53 FRT/FRT; Rosa26 mTmG/+ mice, a single dose of 0.75 mg 4-hydroxytamoxifen in DMSO was delivered via intratumoral injection.
In normal mice a single dose of pegfilgrastim (1000 µg/kg SC) increased the ANC for 5 days, whereas a single injection of filgrastim at a higher dose (2500 µg/kg SC) increased the ANC for only 1 2 days (Fig. 3a).
After the initial tumor was palpated in the Col1a1 FRT-STOP-FRT-Cre-ER-T2; Kras FRT-STOP-FRT-G12D/+; p53 FRT/FRT; Rosa26 mTmG/+ mice, a single dose of 75 mg tamoxifen/kg body weight in corn oil was delivered via intraperitoneal injection.
In Ts65Dn mice, a single dose of a sonic hedgehog agonist SAG 1.1 on the day of birth normalized the cerebellar volume and the deficit in granule cell number to the level observed in euploid littermate controls by P6 (Roper et al., 2006).
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On day 9, control mice received PBS; experimental mice received a single dose of Cy 100 mg kg−1 i.p.
Non-vaccinated control mice received NaCl s.c. at the base of the tail, and the BCG control mice received a single dose of BCG Danish 1331 (2.5×105 CFU) injected s.c. at the base of the tail.
At a dose of 8.1 mg/kg, NITD609 exhibited a potent (but not total) ability to inhibit transmission from mouse to mosquito and completely blocked progression of the life cycle to subsequent mice with a single dose.
For boosting studies, mice received a single dose of rH4 injected s.c. at the base of the tail and two weeks later the mice were given one booster vaccination of Ad-H4 in the right footpad.
Purified carboxylated multiwalled carbon nanotubes (MWCNTs) with different agglomeration propensities were evaluated in mice following a single dose intravenous injection.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com