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"I am a quantification fiend".
(A) Transmission electron microscope showed loosened cell adhesion between astrocytes and vascular endothelial cells in the retrosplenial granular cortex (RSG) of S89G-DMP1 mice; and between astrocytes themselves (B); (C) Representative images of GFAP/lectin in the RSG, indicative of attenuated targeting of astrocytes to blood vessels in S89G mice; (D) Quantification plot for (C).
Statistical analysis: Clinical evaluations of EAE mice and quantification of the pathological features were performed by examiner, blinded to the experimental group.
The serum was also harvested from these mice for quantification of IL-6 (as above) and neutrophil activity using the mouse MPO kit (Hycult Biotechnology) as we previously described [37].
This is because bright fluorescence of melanin granules in C57BL/6 mice hampered quantification of FRET signal and because Fucci mice with FVB/N background were not available.
Only TBS-1 was significantly increased within the adult virgin mammary glands of the p190B+/-Neu mice, and quantification of immunostaining for TBS-1 confirmed this increase.
It has been shown that preclinical ECG-gated perfusion SPECT (in mice) permits quantification of LV volumes and motion as well.
This later effect has been shown to be cell autonomous, as demonstrated by transplantation into the fat pads of immunodeficient mice and quantification of CD31-positive vessels [ 80].
(G ) Dendritic spines on secondary apical dendrites of CA1 neurons of Thy1 EGFP -miR-9-Spfl-Stop (control) and Thy1 EGFP -miR-9-SpNestin-Cre mice and quantification of spine density (n.s, Student's t test, n ≥ 30 neurons per condition recruited from five mice per genotype).
(B ) Frequency of thymocyte subsets in lethally irradiated WT B6 (top left) or RIP-mOVATg (bottom left) recipients reconstituted with T cell-depleted bone marrow from OTII blt /+ and OTII blt/blt mice; quantification of Vα2+ CD4SP thymocytes from indicated RIP-mOVATg chimeric mice (right).
Anti-mouse IgG quantification in the hippocampus revealed a significant increase in 12-month old rTg4510 mice compared to age-matched wild-type mice.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com