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By introducing into mice a modified human TNF transgene lacking a TNF 3' untranslated region involved in translational repression of TNF, it was shown that pronounced TNF overexpression results in chronic polyarthritis with a 100% incidence [ 47].
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LacZ is widely used as a reporter for promoter activity in KO mice and all those mice express a modified protein, whose full function is not known.
Mouse hepatocytes were isolated from livers of 8-week-old mice by a modified two-step collagenase perfusion protocol [34].
They studied a strain of mice with a modified huntingtin gene, which causes them to develop Huntington's-like symptoms.
Bone marrow-derived macrophages were generated from the bone marrow of 8 20-week-old female BALB/c mice using a modified version of the protocol of Lutz et al. (1999).
A high number of shortened tails have been observed in the mice and a modified integument in the tail facilitates tail loss, probably as an anti-predator mechanism.
According to the present results, alteration of cerebellar circuits is able to disrupt the animal's performance in a passive avoidance test, a deficit also reported in Lurcher mice using a modified version of the passive avoidance test [62].
A behavioural deficit was confirmed in the Tg mice using a modified open-field test.
We investigated adolescent mutant mice using a modified three-chamber social arena (Moy et al, 2004).
Ventricular myocytes were isolated from C57Bl/6 mice following a modified version of the AfCS procedure protocol (# PP00000125) (http://www.afcs.org ).org
Neurological function was evaluated in mice by a modified Neurological Severity Scores (mNSS) as well as corner turn and foot-fault tests.
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