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By examining Nestin-GFP expression in transgenic mice, a large number of Nestin-expressing cells were detected as clusters in the renal papilla [108].
Of note, whereas proliferating cells were restricted in the bottom regions of the crypts (stem cell area) in control TNFR1KO mice, a large fraction of proliferating cells was found in the middle or upper region of the crypts in the TNFR1KO/TAK1IEKO mice.
Interestingly, when we analyzed the percentage of Foxp3+ T cells among the Vα3.2-expressing and non-expressing T cells, we found that in single transgenic 2D2 mice, a large proportion of Vα3.2 negative cells express this essential regulatory T cell transcription factor (Fig. 4C).
In tissue-specific mtDNA knockout mice, a large number of apoptotic cells are found in the myocardium.
This time, the results resembled the findings from the initial studies in ApoE−/− mice: a large number of lipid-rich plaques were located in the susceptible aortic regions.
During the generation of the CC mice, a large fraction of the lines ceased to produce offspring leading to an extremely high extinction rate (>80%; F. Pardo-Manuel de Villena, unpublished data).
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Additionally, morphine-tolerant C57BL/6J mice, but not 129P3/J mice, exhibited a large increase in the frequency of tail flicks during the first second following the baseline nociceptive response which may facilitate detection of the response during the tolerant state.
The mean number of Ki67/PDGFRα-positive cells increased after 4 weeks in both the CupR mice) and Cup mice), with a larger number in mice treated with cuprizone (53.1 ± 18.1) than with cuprizone plus rapamycin (27.3 ± 13.4).
Alternatively, this may be a simple statistical effect, given that newly arriving mice would usually be few in numbers and resident mice form a large population.
We found hemosiderin deposition in spleen and enlargement of spleen in F0 and F1 mice, and a large amount of hemosiderin deposition in uterus in F0 mice.
Furthermore, blood analysis demonstrated that male mutant mice had significantly elevated blood insulin compared with controls, with female mice showing a large difference approaching significance (Fig. 4D).
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