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Conventional NOD mice (Kd, I-Ag7, and Db) were bred in our animal facility at the Hôpital Necker as well as CD28−/−NOD mice (a kind gift from J.A. Bluestone, UCSF, San Francisco, CA), CD1d−/−NOD mice (a kind gift from M. Kronenberg, La Jolla institute, San Diego, CA) and IL-4−/−NOD mice (a kind gift from D. Mathis and C. Benoist, Josslin Center, Boston, MA).
Tg2576:sod2 hybrid mice were created by crossing C57BL/6 sod2 null mice (a kind gift of C.J. Epstein) to SJL mice to create F1 C57BL/6:SJL sod2 heterozygotes.
1H9 mAb specificity was tested by using, as a negative control, fibroblasts derived from p130Cas null mice (a kind gift from Dr H. Hirai and T. Nakamoto, Tokyo, Japan).
Ucp2KO mice (a kind gift from Professor Bradford Lowell [21]) were crossed with C57Bl/6J (NntKO) mice to generate heterozygous founders.
Grem1 +/flox mice, a kind gift of E. Canalis, were crossed with Pgk- Cre mice to inactivate one copy of Grem1 in the germline.
Specificity of the anti-BACE antibodies was verified by Western blot using brain homogenates from P7 BACE1−/− mice (a kind gift from Bart De Strooper [ 16]; Fig. S1).
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MEFs were generated from E14.5 embryos of C57BL6 mice, and from p53-knockout mice [ 12]. p53 knock- out mice were a kind gift from Dr Bouillet, Melbourne.
Dermo1Cre/+ mice were a kind gift from Dr. David Ornitz [23], Fgf10LacZ/+ mice were a generous gift from Dr. Robert Kelly [38] and Pitx2−/− embryos were a kind gift of Dr. James Martin [21].
Gadd45a−/− mice were a kind gift from Professor Albert J. Fornace, Jr. and ATM−/− mice were purchased from the Jackson Laboratory.
WT (C57BL/6J) mice were obtained from Jackson Laboratory (Bar Harbor, Maine), Picciotto ("Pic") Chrnb2−/− mutant mice were a kind gift from Dr. M Picciotto [17] and Xu mutants were derived from embryos (ES Cell line ID 00211-UNC) supplied through the Mutant Mouse Regional Resource Center (University of California, Davis, California).
PTEN flox/flox mice were a kind gift from Professor Doreen Cantrell University of Dundee and the MCK-Cre+/− mice were a kind gift from Professor Dario Alessi University of Dundee.
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