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Through zones of inhibition (ZOI) and minimum inhibitory concentrations (MIC) tests, the supernatant of fermentation possessed best antimicrobial activity in all extracts whose MIC value is 31.2 μg/ml against T. rubrum, T. mentagrophytes, S. aureus and P. aeruginosa.
All MIC tests were performed independently in triplicate.
MIC tests were undertaken in 96 flat bottom microtiter plates (TPP, Switzerland).
All MIC tests (alone or in combination) used in this experiment were reference broth microdilution methods in checkerboard tray designs.
Complementary assays involved hemolytic and antifungal MIC tests have been performed to determine selectivity and their biocompatibility with RBC of herbal extracts.
MIC tests were performed in a microtiter plate format as described [12].
Similar(21)
It was shown also that MIC values obtained by fluorescence optical respirometry are consistent with the results of the MIC determinations made by serial dilution method (traditional MIC testing using CLSI).
In the MIC test, the nanofiber mat sample weighing 0.3 mg provides the MIC for all the microorganisms under test.
We would like to thank Tina Mistry for her assistance in performing MIC testing.
Interestingly, a further MIC test demonstrated that the two Gram-negative bacteria were less sensitive than Gram-positive bacteria toward the organic acid-based DESs.
Sodium and potassium chloride salts were separately used for chloride MIC testing, since the counter-ion (potassium or sodium) affects the inhibitory effect of anions (Suzuki et al. [1999]) and therefore the MIC.
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