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To further validate the hits, we performed the broth microdilution MIC test to obtain both MIC and MBC values.
In the MIC test, the nanofiber mat sample weighing 0.3 mg provides the MIC for all the microorganisms under test.
The antimicrobial activity of Ag NPs was studied by Minimum Inhibitory Concentration (MIC) test.
For the minimum inhibitory concentration (MIC) test, the survival of the organisms was observed by visual inspection as recorded [29].
MIC test was performed using sterile 96-well microplates containing 100 µL/well of RPMI-1640 broth.
Finally, the antibacterial activity of the nanocomposites was evaluated using the minimum inhibitory concentration (MIC) test and Kirby-Bauer disk diffusion method.
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It was shown also that MIC values obtained by fluorescence optical respirometry are consistent with the results of the MIC determinations made by serial dilution method (traditional MIC testing using CLSI).
All MIC tests were performed independently in triplicate.
MIC tests were undertaken in 96 flat bottom microtiter plates (TPP, Switzerland).
We would like to thank Tina Mistry for her assistance in performing MIC testing.
All MIC tests (alone or in combination) used in this experiment were reference broth microdilution methods in checkerboard tray designs.
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