Exact(3)
The minimum bactericidal and fungicidal concentration (MBC and MFC) was determined by the viable cell count method [23] by subculturing 50 μl of each dilution used in MIC experiment.
To assay the MBC, 100 µl cultures was taken from each well in the above MIC experiment, centrifuged and washed three times with PBS and plated on a LB agar plate separately, and bacterial cells were enumerated after incubation at 37°C for 24 h.
MBC values were determined by removing 100 μL of bacterial suspension from culture demonstrating no visible growth in MIC experiment and inoculating in nutrient agar plates.
Similar(57)
In these control studies, a bacterial population (106 CFU) which is typically used in MIC experiments was incubated in the presence of different antibiotic concentrations.
Bacteria were grown in Luria bertani (LB) broth (Difco) except during MIC experiments, in which Muller Hinton (MH) broth (Difco) was used.
CLSI MIC experiments were repeated three times in triplicate.
Antimicrobial minimum inhibitory concentrations (MIC) experiments with Nu-3 and controls were measured against a range of Gram-positive and Gram-negative bacteria, including some hospital isolates according to Clinical and Laboratory Standards Institute (CLSI) guidelines.
All other MIC and MBC experiments were repeated on three different occasions, and a fourth test was performed if the values were not identical.
Monontonically decreasing activity was observed with increased PEG spacer length in solution-based MIC assays; however, experiments with polymerized vancomycin derivatives suggest that PEG tethers may be useful for separating the antibiotic moiety from other camouflaging polymer architectures and for spatially enabling desired biochemical interactions.
In conclusion, CTE exhibited antifungal activity against P. litchii in vitro experiment, with MIC and MFC values of 5 and 10 mg/L, respectively.
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