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The starchy endosperm samples (500 mg) were powdered in liquid nitrogen and suspended in 1 mL of 10 mM Tris HCl (pH 7.5), 1.0 mM CaCl2 and 0.1%% (w/v) Triton X-100 for 15 h at 4 °C.
Tumor samples (20 to 30 mg) were powdered under liquid nitrogen.
The plant materials (200 mg) were powdered in liquid nitrogen.
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The photocatalyst (80 mg) was powdered and added to the vessel containing 80 mL 3 × 10−5 mol L−1 MO or p-hydroxyazobenzene (1.2 × 10−4 mol L−1) aqueous solution.
In brief, each sample (∼3 mg) was powdered and bleached for 48 h with 12% NaOCl.
Frozen plant tissue (400 mg) was powdered in liquid nitrogen and extracted with methanol (4 ml per g-1 fresh weight), heated for 15 min at 70°C, and centrifuged (5 min., 12000 g).
Briefly, cardiac tissue (50 mg) was powdered in liquid nitrogen and extracted with RIPA buffer containing protease inhibitors, followed by centrifugation (10,000 rpm for 10 min) and recovery of the supernatant.
Briefly, cardiac tissue (50 mg) was powdered in liquid nitrogen and extracted with Radio-immunoassay Precipitation Assay (RIPA) buffer containing protease inhibitors, before centrifugation and recovery of the supernatant.
Adipose tissue (250 mg) was powdered on dry ice and homogenized in 300 μl lysis buffer (50 mM HEPES (pH 8), 150 mM NaCl, 1% Triton X-100, 1 mM Na3VO4, 30 mM NaF, 10 mM Na4P2O7, 10 mM EDTA with protease inhibitors (set III, Calbiochem Novabiochem Biosciences, Cambridge, UK)).
Ten tablets of Exviera®tablet (containing 250 mg DAS) were powdered, and an amount equivalent to 10 mg of DAS was accurately weighed into a 10-ml volumetric flask and mixed with 10 drops of DMSO.
Twenty tablets of Sectral® (Alexandria Pharm. & Chem. Ind. Co., Egypt) labeled to contain 200 mg AC per tablet were powdered.
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