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Freeze-dried mycelia (10 mg) were crushed using mortar and pestle.
Pieces of frozen needles (around 30 - 40 mg) were crushed using a mixer mill (Retsch MM300, Haan, Germany).
Pieces of frozen needles (around 30 to 40 mg) were crushed using a mixer mill (Retsch MM300, Haan, Germany).
Frozen samples (400 mg) were crushed into powder and extracted separately with 2 ml of pH 1.0 buffer containing 50 mM KCl and 150 mM HCl as well as with 2 ml of pH 4.5 buffer containing 400 mM sodium acetate and 240 mM HCl.
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Frozen tissue (25 50 mg) was crushed into a fine powder with a pestle and mortar, followed by RNA extraction using RNA-Bee™ Biogenesis Ltd, Poole, UK, according to the manufacturer's protocol.
Frozen cartilage (200 mg) and bone (150 mg) samples were crushed in liquid nitrogen using a hammer and a stainless steel mortar and pestle and then ground to a fine powder in liquid nitrogen using a porcelain mortar and pestle.
Curcumin microsponges [100 mg] were crushed and extracted using 10 mL methanol by vortexing and centrifuging at 2000 rpm for 10 min.
To extract total protein, 200 mg of frozen biopsies were crushed with a mortar and pestle in liquid nitrogen.
Approximately 100 mg of plant tissues were crushed in liquid nitrogen with poly-vinyl-poly pyrrolidone.
500 mg of these spores were crushed using liquid nitrogen and then the powder was suspended in 30 ml of Cell Suspension Solution.
Akineton tablets (containing 2 mg Biperiden; producer: Abbott Laboratories) were crushed and mixed with conventional pig food, honey (to make it more palatable), and some water into a ball.
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CEO of Professional Science Editing for Scientists @ prosciediting.com