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Triplicate sediment samples (~250 mg) were collected sterilely, immediately frozen on dry ice, and transported back to the laboratory.
For initial screening, six leaf discs (~11 mg) were collected from different positions in transfected leaves, ground in 200 ul 50 mM phosphate buffer, centrifuged and assayed for AChE levels from days 3 to 16. Leaves were then either directly harvested and processed or stored at −20 °C; a small 200 g batch of leaf biomass typically producing ~70 mg of rAChE.
Compounds 1 (23.1 mg) and 2 (14.7 mg) were collected after semi-preparative HPLC (Shimadzu LC-8A) on an XTerra® Prep.
Hair samples (50 mg) were collected from 233 volunteers as part of a research project investigating the discriminatory power of drug- and doping-related explicit and implicit social cognitive measurements in behavioural context indexed on the presence or absence of the target drugs in hair.
Mouse feces (about 100 mg) were collected, vortexed in phosphate-buffered saline (PBS), and centrifuged for 10 min at 800 rpm.
Young leaves (500 mg) were collected and kept at −80°C until RNA isolation.
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Lyophilized microalgae powder (about 20 mg) was collected for transesterification.
At the same time, unreacted -compound 6 -Ves-OH) (50.2 mg) was collected as a yellow powder.
On the day of sacrifice, a fresh sample of each colon (50 mg) was collected for myeloperoxidase (MPO) assay according to Krawisz et al., [27].
Each soil sample of about 500 mg was collected from the 0 15 cm layer, which represented the plough layer.
Mammary parenchymal tissue (30 50 mg) was collected with a needle biopsy (Bard® Magnum® reusable core biopsy gun and 12G, 10 cm core biopsy needles, Bard Biopsy Systems, AZ, USA) as described by Norgaard et al. [ 34].
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