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Since in long-term studies usually 80 mg were applied, we also decided to use this dosage.
Polysaccharide samples (100 mg) were applied to a DEAE-cellulose column (40 ×2.5 cm), equilibrated with 0.01 M sodium acetate (pH 5.0), and eluted by with a linear gradient of 1 3 M NaCl in the same solution.
The sulfated polysaccharide samples obtained from the DEAE-cellulose column (10 mg) were applied to a MonoQ column coupled with an FPLC system, equilibrated with 20 mM Tris HCl (pH 8.0) containing 10 mM ethylenediamine tetra-acetic acid.
After adequate rewarming, the propofol dose was stopped, single doses of pethidin 25 mg and piritramid 3.75 mg were applied, and the remifentanil dose was continuously reduced.
Protein samples (2 mg) were applied to IPG strip (17 cm, pH3 10 NL, Bio-Rad) using a passive rehydration method.
On the 2nd day of induction, initially 2 mg and after 6 to 8 hours another 1 mg were applied if no regular contraction was felt by the patient.
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For gravimetric observation of dehydration process in prepared samples, the analytical balance with the accuracy equal to 0.1 mg was applied.
Cortex moutan heteroglycan (500 mg) was applied to a column (3.2 cm × 32 cm) of DEAE Sepharose CL-6B (Cl−) that was equilibrated with H2O.
A tension of 1 mg was applied for each segment.
Pazopanib 800 mg was applied continuously once daily.
However, such limitations are undesirable when MG is applied to longer genomic sequences for precise annotations.
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