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% Mg were analyzed using X-ray photoelectron spectroscopy.
Concentrations of K, Ca and Mg were analyzed after high-pressure digestion with nitric acid (UltraClave III, MLS, Leutkirch, Germany) using inductively-coupled plasma optical emission spectrometry (ICP-OES 720, Varian, Palo Alto, CA, USA).
Samples with masses between 1 10 mg were analyzed using platinum pan.
Cations such as Na, K, Ca and Mg were analyzed using atomic absorption spectrophotometer, Shimadzu-AA-6800.
Cured samples (50 mg) were analyzed in open (silicon) pans at 20 K/min in N2 atmosphere.
Aluminum was analyzed by titrating this mixture with 0.015 mol L−1 NaOH and bromothymol blue indicator, and Ca and Mg were analyzed using an atomic absorption spectrophotometer.
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MG was analyzed in different water samples (urban, mineral and river waters) using the proposed method.
Compound 5 (0.010 mg) was analyzed using a Gemini (Phenomenex, Torrence, CA) 5u reverse phase C18 column (250×4.6 mm) to chromatograph these molecules with a 0 to 100% gradient of 0.1M tetraethylammonium acetate (Aldrich), pH 6.5 and acetonitrile (Aldrich) at a flow rate of 1 mL/min.
About 0.8 to 1.2 mg was analyzed for carbon and nitrogen determinations.
All MGs were analyzed by Northern blotting (data not shown), Western blotting (Fig. 2A) and immunohistochemistry (Fig. 2B and data not shown).
In total, 63 MGs were analyzed (non-historical singleton MGs were excluded from the network for simplicity see supplemental information) and the MGs of the historical isolates were then located on the network.
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