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Sugar beet arabinan (20 mg) was treated with PoAbf62A for 12 h in conditions described above.
Compound 2 (0.5 mg) was treated with - R -MTPA-Cl (15 μL) in pyridine (0.2 mL) at room temperature for 16 h.
Freshly prepared powder (30 mg) was treated with 1.5 mL of 1-octadecene at 150°C for 16 h under magnetic stirring in N2 atmosphere.
Compound 1 (2.0 mg) was treated with 3 M HCl for 4 h at 100 °C, and the solution was then neutralized with sodium bicarbonate and extracted thrice with EtOAc.
Genomic DNA (5 mg) was treated with Cre recombinase, phenol extracted, ethanol precipitated, and used to transform DH10B-lac-trfA.
Each sample (250 mg) was treated with H2SO4 (72% mass) at 30°C for 1 h.
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Twenty-four of 25 PRSP-infected patients receiving amoxicillin/clavulanate, 2000/125 mg were treated successfully.
The heterogeneity in the diopside is mostly due to Fe2+ Mg exchange, and it does not affect our analysis because Fe2+ and Mg are treated as a single component (FM).
In an independent experiment, samples (10 mg) were treated with trifluoroacetic acid (TFA) (at 0.1% final concentration in the sample) to extract the peptides adsorbed on different proteins, chaperones and albumin.
Tissue fractions (200 mg) were treated with acetonitrile (2 mL) and homogenised, and the mixture was centrifuged at 2,000 rpm.
Proteins (0.4 mg) were treated with 10 mU endoglycosidase H or 125 mU endoglycosidase F at 37°C for 16 h.
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