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A homogenized representative sample (10 mg) was transferred to a 10 mL volumetric flask and made up to the mark with mobile phase with intermittent shaking.
At 5 min after injection, the valve was switched into position A. FA (2.5 mg) was transferred to a 200 mL conical flask together with 150 mL distilled water.
Each sample (50 200 mg) was transferred to pre-weighted 9 mm large bulb glass sample cell and degassed at 200 °C under vacuum for 24 h on XeriPerp degasser (Quantachrome Instruments) which is equipped with a turbo molecular vacuum pump and controlled heat jackets.
Hemin (0.8 mmol, 500 mg) was transferred in an inert atmosphere box.
For a second approach to measure polyphenol contribution, Acai-PS (100 mg) was transferred over a column containing 2 g of triple-washed PVPP.
Dust was baked at 50°C overnight, sifted through chessecloth mesh, and 30 mg was transferred to a vial for each assay.
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Approximately 50 ~ 100 mg of the fecal sample were transferred into a pre-weighed tube by means of an inoculation loop.
Microspheres of 100 mg were transferred to each plastic tube containing pre-warmed dissolution media (10 ml) and maintained at 37 ± 0.5 °C under agitation with 25 oscillations/min on gel rocker at 25 30° angle of inclination.
Liver slices (100 mg) were transferred into clean sample collection glass vials filled with KRHB (3.0 ml).
The tissue punches (4 mg) were transferred to XF24 Islet Capture Microplate (Seahorse Bioscience, North Billerica, MA) and a screen was loaded on to each well.
Two-week-old A. thaliana Col-0 seedlings (600 mg) were transferred to liquid ½ MS medium and compound 15 was added to a final concentration of 50 μM.
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