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Colonic tissue (50 mg) was processed for reduced glutathione (GSH) analysis by HPLC [21].
The same amount of protein (1 mg) was processed in an equal volume of buffer for all samples.
An average of 152.74 ± 21.01 mg was processed, and after digestion, 5.56 ± 1.54 × 10 cells were obtained and in vitro seeded.
For quantification of triacylglycerides, the biomass was lyophilized and weighed, and 1 mg was processed for total lipid extraction as described by Bligh and Dyer [ 44]. 10 μg of cetyl palmitate was added before extraction as an internal standard for load control.
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HPLC chromatograms of the standards solution and sample (under the optimal conditions) are shown in Fig. 7. To determine the repeatability of the novel extraction method, six samples of the same weight (10 mg) were processed under the optimum extraction conditions.
Fecal samples (200 mg) were processed using the FastDNA SPIN Kit for Soil (MP Biomedicals), incorporating a bead-beating step for mechanical disruption of cells.
Best results were obtained when cryosections were prepared in <20 min. Cryosections, 7 μm and 10 μm, respectively, for MA and MG, were processed without staining and epithelial and stromal cells were easily visualized on the LCM instrument via contrast adjustment of the microscopic image.
The method was capable of detecting (LLOD) stanozolol and 3′-hydroxystanozolol in hair at concentrations as low as 0.125 pg/mg and 0.25 pg/mg respectively when ca. 50 mg hair was processed.
100 180 mg of tissue was processed per sample, according to the manufacturer's recommendations.
Approximately 25 mg of tissue was processed following the standard protocol of a DNeasy Blood & Tissue Kit QIAGENN, Hilden, Germany).
Approximately 100 mg of sample was processed with PowerSoil DNA isolation kit (MoBio Laboratories Ltd ,Carlsbad, CA) to isolate genomic DNA.
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