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To emulsify the pheomelanin, a small quantity (~0.1 mg) was placed in a 2 mL microcentrifuge tube, to which 1 mL H2O and 0.25 mL hexane were added.
Briefly, samples of adipose tissue (250 mg) was placed in 2.5 ml of ice-cold SHB buffer (20 mM Tris, 1 M sucrose, 1 mM EDTA) containing protease and phosphatase inhibitor cocktail, as well as Triton-X100 (Sigma).
Solid C60 (1.5 mg) was placed between two glass microscope slides (76 mm × 26 mm, thickness 0.8 – 1.0 mm) and rubbed repeatedly between fingertips for a few minutes (Fig. 1a).
A sample of 300 mg was placed in the Sievert apparatus and washed with argon gas.
The solid sample (few grains, <0.1 mg) was placed on the rhenium filament of Direct exposure probe (DEP).
For the ASC measurements, a sample of 80.0 mg was placed inside a Mettler Toledo 120 (upmu mathrm{l}) stainless steel Medium Pressure Crucible.
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Samples of Sali-NF (10 mg) were placed in 2 ml microfuge tubes and were incubated in 1 ml PBS under shaking conditions (60 rpm).
(6, 5 -enriched SWCNTs (1.0 mg) were placed in a water solution (10 mL) of fullerodendron (25 -enriched1 mmol) and then SWCNTsted with a bath-type ultrasonic cleaner (Honda Electronics Co., Ltd., vs-D1.0, 110 W, 24 kHz) at 17–25 °C for 4 h.
Clinoptilolite samples (grain size 0.20 0.31 mm) weighting 500 510 mg were placed in corundum crucibles.
The electospun mats, of each 50 mg, were placed into 5 mL bacterial solution.
Drug-loaded nanofibers (200 mg) were placed in 900 mL of physiological saline (PS; 0.9 wt%) at 37°C ± 1°C.
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