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Tuber tissue was powdered in liquid nitrogen, and 100 mg was extracted with 100 μL of extraction buffer (50 mM HEPES-NaOH, pH 7.4, 2 mM MgCl2, 50 mM 2-mercaptoethanol, 12.5% (v/v) glycerol), incubated on ice for 5 min then centrifuged twice at 16,000 × g for 15 min [ 17].
A powdered sample (200 mg) was extracted in solvent (methanol: H2O = 8 2) by reflux extraction method at 80°C three times, and concentrated to 50 mL.
A small subset of sample (~100 mg) was extracted from the mesh bag, sprinkled onto the grid, and gently pushed into the grid surface to secure the sample prior to loading the stub into the microscope.
After removal of the solvent, the residue (960 mg) was extracted with dichloromethane at room temperature.
Genomic DNA from fecal samples (50 100 mg) was extracted using a FastDNA SPIN kit for soil (MP Biomedicals, CA, USA).
A sample of the honey (100 mg) was extracted with 10 mL of 1% metaphosphoric acid at room temperature for 45 min and filtered through Whatman No. 4 filter paper.
Similar(29)
(a) Direct extraction with KOH Dried ground manures (500 mg) were extracted with 20 ml of 1N KOH, for 1 h at 105 °C, in sealed Pyrex tubes placed in a heating block.
Aliquots of frozen powder (100 mg) were extracted based on a modified method used in Bowne et al. (2012).
Different rice bran samples (50 mg) were extracted with methanol/HCl (99 1 v/v) solute on at 4 °C for overnight.
Exchangeable K, Na, Ca, and Mg were extracted by leaching the soil with neutral ammonium acetate (NH4OAC).
Ca and Mg were extracted by ammonium acetate method (Peech et al. 1947) and determined in FLAAS with the detection limit 0.50 and 0.3 mg/L, respectively.
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