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The terminal tumor weight (mg) was determined and applied to evaluate the antitumor effects.
Cation like as Ca and Mg was determined by titration method with 0.01 N EDTA (Klute 1986) and sodium (Na) and potassium (K) were measured by flame photometry.
Reusability of laccase nanoparticles (100 mg) was determined by incubating it with ABTS in Na-acetate buffer at 30 °C for 3 min.
On the basis of optimization criteria, composition of optimized formulation F0 (Methocel K100LV = 77.98 mg and Carbopol 934 = 82.02 mg) was determined by statistical analysis.
Concentration of the protein (mg) was determined using Lowry method with bovine serum albumin as standard (Lowry et al. 1951) and specific activity (IU/mg) using enzyme units and protein concentration were calculated.
Also the amount of soluble cations (Ca, K, Na and Mg) was determined by FAAS method showing high amount of calcium, potassium and sodium in the Haft Tappeh samples (Table 5).
Similar(36)
HCO3, Cl, Ca and Mg were determined by titration.
Ca, Na and Mg were determined by Instrumental Neutron Activation Analysis (INAA).
Ca and Mg were determined by EDTA titration (Allen and Hajek 1989).
The contents of P, K, Na, Ca and Mg were determined by atomic absorption spectroscopy (Shimadzu, AA-6200).
Na and K in the water samples were determined by a flame photometer (Systronics, Germany), whereas TH, Ca, and Mg were determined by ethylenediaminetetraacetic acid titrametric methods.
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