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Cortex moutan heteroglycan (500 mg) was applied to a column (3.2 cm × 32 cm) of DEAE Sepharose CL-6B (Cl−) that was equilibrated with H2O.
For gravimetric observation of dehydration process in prepared samples, the analytical balance with the accuracy equal to 0.1 mg was applied.
A dialyzed sample (5 mg) was applied to a SephadexTM G-75 (Sigma Chemical Com., St . Louis MO, USA) column (1.2 × 42 cm) and eluted using 0.1 M Tris HCl pH 8.0 at a flow rate of 0.34 mL min-1 at room temperature.
A tension of 1 mg was applied for each segment.
Pazopanib 800 mg was applied continuously once daily.
Bleomycin 15 mg was applied over 15 min on days 1 and 8.
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Since in long-term studies usually 80 mg were applied, we also decided to use this dosage.
Polysaccharide samples (100 mg) were applied to a DEAE-cellulose column (40 ×2.5 cm), equilibrated with 0.01 M sodium acetate (pH 5.0), and eluted by with a linear gradient of 1 3 M NaCl in the same solution.
The sulfated polysaccharide samples obtained from the DEAE-cellulose column (10 mg) were applied to a MonoQ column coupled with an FPLC system, equilibrated with 20 mM Tris HCl (pH 8.0) containing 10 mM ethylenediamine tetra-acetic acid.
However, such limitations are undesirable when MG is applied to longer genomic sequences for precise annotations.
When MG is applied to very short sequences containing one or two partial genes, these limitations are not significant.
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