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Separate 25 mg pieces of the same muscles were used to isolate the total mixed protein and tissue-free fluid fractions [25] [27].

Briefly, 30 50 mg pieces of frozen liver were homogenised in lysis buffer (10 mM Tris pH 8, 0.2% (v/v) NP-40, 1x complete protease inhibitors (Roche)) using a TissueLyser (Qiagen) and cleared by centrifugation.

Porcine cardiac tissue served as a positive control for N-cadherin immunohistochemistry. Approximately 3 4 mg pieces of each construct (n = 6 per time point) were frozen for biochemical analysis in both phases.

For mutations I736T, R719W and V606M, total RNA was isolated from approximately 3 mg pieces of frozen muscle using Trizol (GIBCO, Karlsruhe, Germany).

The maximum recommended dosage per day was 15 x 4 mg pieces of gum or 40 x 2 mg tablets for the high-nicotine dependent group, and 15 x 2 mg pieces of gum or 20 x 2 mg tablets for the low-nicotine dependent group.

HPLC analysis: For NAD+ extraction, 500 μl of 1.66 M HClO4 was added to the 4 10 mg pieces of frozen tissues and homogenized in CKMix tubes (Precellys), thrice, for 20 s each, at 4,000 rpm of homogenizing speed in Precellys homogenizer with 2-min breaks on ice.

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Similar(53)

A 50 mg piece of 99.995 + % (metals basis) pure Fe was placed in the 100 μL Alumina crucible.

For the calibration of the heat capacity, a 12 mg piece of sapphire was placed in a sample pan and measured at 10 K min(^{-1}).

A small (approximately 30 mg) piece of the large lobe was set aside for QNMR composition measurements.

For each individual, total RNA was extracted from a 20 30 mg piece of liver using the RNAeasy Mini Kit (Qiagen).

In all, 300  μl of lysis buffer was rapidly added to the tube for a 5 mg piece of tissue and homogenized with an electric homogenizer.

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