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Thirty mg of tissue were used for protein content analysis.
100 180 mg of tissue was processed per sample, according to the manufacturer's recommendations.
Mean total RNA yield was 90±130 µg per mg of tissue.
Total RNA was isolated from 30 mg of tissue using TRIzol (Invitrogen) and DNase treated (Ambion).
Results are expressed as pg/ml or pg per 100 mg of tissue.
About 25 mg of tissue was then cut off and set aside for RNA extraction.
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CPA was also found in the olfactory bulb at concentrations around 0.1 ng/mg of tissue.
Another treatment-naı̈ve patient with a history of high HBV viral load had 1×105 copies of cccDNA/mg of tissue (4×106 copies of tDNA/mg of tissue).
However, maximal brain concentrations of the active compound DB75 were very low (13 nmol/mg of tissue at 24 h).
Furthermore, peak DB75 tissue levels were higher (61 nmol/mg of tissue at 24 h) than with oral prodrug.
Results were expressed as ng 11-KT/mg of tissue.
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