However, in most comparative studies, roughly 100 mg of the specimens were used.
Brown (1973) gives a venom yield range of 200 1000 mg (of dried venom), A range of 200 600 mg for specimens 125 155 cm in length has also been reported.
Specimens were tested for WNV RNA by using TaqMan and standard RT-PCR (2 ) with minor modifications; 100 mg of each specimen was diluted in 1.0 mL of BA-1, homogenized, centrifuged, and sterile filtered.
Approximately 25 mg of brain specimen from each animal was homogenized, and 1 mL of TRIzol reagent (Invitrogen, Carlsbad, CA, USA) was added.
Total RNA from microscopically confirmed homogeneous breast cancer tissue was isolated from approximately 500 mg of frozen specimen or from one flask of the HepG2 cell line, kindly provided by Dr N Harada [ 21], as a positive control and to generate standard curves.
Next , 25to 30 mg of muscle tissue specimens were weighed on an aluminum sheet using an analytical balance and transferred to Eppendorf tubes (1.5 to 2 ml).
15 20 mg of tissue bank specimens were placed into 700 μL of RNA extraction lysis buffer (Qiagen) and then homogenized using a PRO 200 rotor stator homogenizer (PRO Scientific Inc Oxfordd, CT).
For the rolled Mg alloy, three types of specimens, whose longitudinal axes are parallel (R specimen) and perpendicular (T and S specimens) to the rolling direction, were prepared.
Participants were given albendazole 400 mg after the collection of specimens.
Total RNA was isolated from 100 mg of the tumour specimen using TRIzol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.
