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The resulting cell-free extracts (CFEs) typically contain >1 mg of soluble Sav.
In those who took 10 mg of Zocor daily plus 5 mg of soluble fiber in the form of psyllium (Metamucil) three times a day, LDL levels dropped 36% — the same as seen with twice as much Zocor.
In batch cultivations, the highest specific P2O activity of 0.9 U (mg of soluble protein)−1 was measured in oxygen-limited cultures grown at 25 °C.
After simple purification steps including denaturation and refolding, 87.7 mg of soluble FcγRIIa that was more than 95% pure was obtained from a 20-mL culture with high recovery yield (≈54%).
From styA expression, a cell dry weight of 1.2 g and 7.8 mg of soluble StyA protein per liter culture was obtained.
We collected 5.14 g (wet weight) of U. thermosphaeicus cells from 500 ml of culture medium, and then obtained about 260 mg of soluble protein from the crude cell extract after sonication and centrifugation.
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Elution of the bound proteins using 500 mM imidazole resulted in highly purified rMEHB rendering 1.5 mg from 25 mL of soluble protein obtained after cells lysis.
From a nutritional point of view, higher sweetness (from 113 to 149 mg g−1 of soluble sugars in dry matter) and an improved P, K and Mg content of bulbs (4.00, 11.65 and 3.18 g kg−1, respectively) in response to HS addition has been ascribed.
The single dose (40 mg) of the soluble t-Res was well absorbed and elicited biologically efficient blood levels (0.1 6 μM) for several hours, despite metabolization into glucuronide and sulfate conjugates coupled to renal elimination.
P22sTsp expression yielded 25 50 mg of purified soluble protein per litre of bacterial culture.
For the immunoprecipitation experiments, approximately 6 mg of total soluble protein extract (∼10 mg/ml) was then incubated for 1 hour at 4°C with anti-FLAG M2 Agarose affinity beads (Sigma-Aldrich) for F/H tagged strains, or with IgG Sepharose 6 Fast Flow beads (Amersham Pharmacia) for TAP-tagged strains.
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