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The purified libraries with approximately 20 mg of small RNA were used for cluster generation and sequencing analysis using the Solexa sequencer (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.
In brief, 20 mg of small (∼70 nm) or large size (∼150 nm) PLGA nanoparticles was added to approximately 1 mCi of [F]NPB4 in 0.5 mL of PBS.
For RT reactions, 1 mg of small RNAs was reverse transcribed with the miScript Reverse Transcription Kit (Qiagen, CA, USA) at 37°C for 60 min followed by a final incubation at 95°C for 5 min.
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(226) With modern NMR instruments it is possible to perform thorough investigations on amounts as small as 1 mg of a small oligosaccharide.
A minimum of 40 mg for small lesions involving less than half the diameter of the involved bone, and up to 160 mg for large lesions of the pelvis has been recommended [36, 37, 38].
MGs are composed of small power sources which can be renewable, placed near customer sites.
These results show that the 6c sites are composed of La and Mg with small fluctuations of the La/Mg ratio (within the errors bars).
10 mg of each adhesive in small pieces and introduced in the TGA equipment.
After 2 washings in RNase-free water, 20 mg of tissue excised in small pieces were homogenized in RLT solution with tungsten beads and using the Mixer Mill MM300 (Qiagen).
When the IE calculation was done, nearly twice the amount of large islet volume was needed to obtain 10 mg of protein, compared to small islets.
For the EMSA experiments shown in Figure 4: (A) Control 'nuclear extracts' correspond to 1 μl of 0 6 hr nuclear extracts (corresponding to about 2.5 mg of embryos) from the small scale preparation described above.
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