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About 10 mg of samples was pretreated at 120 °C for 1 h under nitrogen atmosphere at 100 ml/min.
Latex solutions were prepared by dissolving 120 mg of samples in 4 ml of different pH value solutions.
Approximately 200 mg of samples were loaded into the sample holder, taking care not to introduce preferred orientation of the crystals.
Approximately 100 mg of samples was pulverized with liquid nitrogen in a mortar and then extracted by DNeasy® Plant Mini Kit (Qiagen, Germany).
The KBr discs were made by pressing the mixture, which contained 10 mg of samples with 100 mg of KBr, at a pressure of 125 kg/cm2.
Approximately 10 mg of samples was incubated with 30.9 μl of 20 mg/ml proteinase K (Invitrogen, AM 2546) in 340 μl 50 mM HEPES.
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For each run, about 15 mg of sample was used.
Only about 10 mg of sample material was required.
The KBr pressed disc technique (1 mg of sample and 160 mg of KBr) was used.
The limit of detection was 0.06 μg g−1 (wet mass) for 30 mg of sample.
After weighing, ca. 20 mg of sample was transferred to a centrifuge tube.
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